LI-750 DIGITAL PCR SYSTEM

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Description

SYSTEM OVERVIEW

A complete, fully integrated Digital PCR platform combining in-built partitioning, thermal cycling, and imaging modules in a single walk-away automated system. Designed for minimal hands-on time, reduced human error, ease of use, and simplified maintenance. Delivers precise, multiplexed absolute quantification results under 3 hours.

KEY FEATURES & BENEFITS

Complete Walk-Away Automation

  • Integrated partitioning + thermal cycling + imaging in one platform
  • Minimal hands-on operation from sample to result
  • Compatible with front-end automation for automated PCR setup
  • User-friendly plate setup identical to standard qPCR workflows

High-Performance Multiplexing

  • Up to 6 detection channels (excluding any reference channel)
  • Multiplex quantification of up to 12 target DNA or RNA molecules per assay
  • Saves time and reagents while maximizing data per run

Advanced Optical System

  • LED-based illumination
  • High-sensitivity CMOS camera for image acquisition

Precision Partitioning Technology

  • Automated partitioning platform generating fixed-size partitions
  • Eliminates partition size variation
  • Partition range: 8,500 up to 26,000 partitions
  • Supports 8-, 24-, or 96-sample configurations per run
  • Minimum 90% valid partitions per reaction well

Superior Quantification Accuracy

  • Volume Precision Factor (VPF) technology for exact and reproducible concentration calculations
  • Highest precision of concentration results

Chemistry Compatibility

  • Fully compatible with probe-based detection chemistry
  • Compatible with EvaGreen chemistry
  • Validated Master Mixes supplied by vendor

APPLICATIONS

  • Mutation detection
  • Copy Number Variation (CNV) analysis
  • Gene expression studies
  • Gene-editing analysis
  • Pathogen detection

THROUGHPUT & FLEXIBILITY

ParameterSpecification
Maximum Throughput96 reactions simultaneously
Sample Formats8-well (low) / 24-well (mid) / 96-well (high)
Plate LayoutANSI/SBS standard format
Partition Flexibility8,500 – 26,000 partitions (selectable)
ScalabilityInstrument and plate configurations adaptable to throughput needs

SOFTWARE SUITE

Comprehensive software package enabling:

  • Assay design
  • Run setup and monitoring
  • Data access and analysis
  • Full data processing and result generation in under 3 hours

TECHNICAL HIGHLIGHTS SUMMARY

  • Fully integrated partitioning, thermal cycling & imaging modules
  • Walk-away automation with minimal hands-on time
  • Up to 6 optical channels → up to 12-plex capability
  • LED + CMOS imaging
  • Fixed partitions (8,500–26,000)
  • ≥90% valid partitions
  • VPF for highest concentration precision
  • Probe-based + EvaGreen chemistry support
  • Vendor-validated Master Mixes
  • Front-end automation compatible
  • ANSI/SBS plate format
  • Scalable from 8 to 96 samples per run
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