Fluorescent Microscope (AFM 100)

The AFM-100 Fluorescent Microscope is a high-performance instrument engineered for advanced clinical, research, and diagnostic laboratories. It delivers bright, high-resolution fluorescent imaging through long-working-distance plan achromatic objectives and a precise, stable optical system. Compatible with blue, green, and UV excitation, it supports a variety of fluorescent stains. The trinocular head enables simultaneous viewing, imaging, and documentation. Equipped with a smooth mechanical stage, coaxial fine and coarse focusing, and a robust HBO mercury lamp system, the AFM-100 is ideal for cell biology, immunology, microbiology, and disease diagnostics, ensuring accurate and reliable results every time.

Description

AFM-100 Fluorescent Microscope – Technical Specifications & Features

Technical Specifications

Specification Details
Model AFM-100
Viewing Head Compensation-free trinocular, 30° inclined
Interpupillary Distance 48–75 mm
Eyepiece EW 10X / 20 mm wide-field
Nosepiece Inverse quadruple
Objectives 4X/0.10, 10X/0.25, 20X/0.65, 40X/1.25 (fluorescent)
Optical System Infinite plan achromatic
Stage Double-layer mechanical stage, 210 × 140 mm, travel 75 × 50 mm
Focusing Coaxial coarse and fine, 2 µm minimum fine adjustment
Transmitted Light Source 6V / 20W halogen lamp
Fluorescence Lamp 100W HBO mercury lamp
Filters Blue, green, UV, frosted glass
Power Supply AC 220V
Centering Telescope 30 mm
Immersion Oil Included
Safety UV protection barrier

Key Features

  • Delivers high-resolution fluorescent imaging for detailed observation.

  • Infinite optical system ensures sharp, bright, and distortion-free images.

  • Wide-field EW 10X/20 mm eyepiece for clear visualization.

  • Trinocular head inclined at 30° allows comfortable viewing and camera attachment.

  • Plan achromatic objectives with long working distance for precise focusing.

  • Blue, green, and UV excitation filters for versatile fluorescence applications.

  • Inverse quadruple nosepiece enables rapid objective changes.

  • Coaxial coarse and fine focusing system with 2 µm precision for accurate positioning.

  • Double-layer mechanical stage (210 × 140 mm) with 75 × 50 mm travel for stable slide handling.

  • Halogen lamp (6V/20W) for transmitted light observation.

  • 100W HBO mercury lamp for bright fluorescence excitation.

  • UV protection barrier ensures user safety.

  • Sturdy, reliable frame for extended laboratory use.


Working Principle

The AFM-100 Fluorescent Microscope works by directing light from the mercury lamp through the selected excitation filters onto the specimen. Fluorescent dyes in the sample absorb this light and emit visible fluorescence, which passes through barrier filters before reaching the eyepiece or camera port. The infinite optical system produces bright, clear, and sharp fluorescent images. The mechanical stage and fine focusing allow precise positioning and optimal focus for accurate observation and imaging.


Applications / Use Cases

  • Cell culture research and monitoring of live samples.

  • Fluorescent staining in microbiology and immunology studies.

  • Clinical diagnostics in pathology and immunology labs.

  • Research laboratories using blue, green, or UV fluorescence techniques.

  • Identification of viruses, bacteria, and microorganisms.

  • Medical and educational institutions for teaching and practical demonstrations.

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